Active ingredients enhancement by A.rhizogenes in Hyoscyamus muticus von Alia/Abu El-Ella Nesiem Amer

1st part of this study, the optimized protocol for induction of Egyptian henbane hairy roots is to use excised leaf explant had 2 months age and infected with A. rhizogenes strain LBA 9402/12 at intensity corresponding to OD600 = 0.6 by using the infection method normal dipping for 30 min. combined with acetosyringon (500 ¿M). Leaf explants were incubated on MS medium for 2 days under dark condition. Then, transferred to MS medium containing 500 mg/l of rifampicin and incubated for 16 days under 16/8 h (day/night). Leaf explants infected with LBA 9402/12 were cultured on MS and incubated on 25ºC at day/night conditions for 16 days produced significant higher response for hairy root and it has a longest growth curve in growth kinetic study. 2nd part, the highest callus fresh weight was obtained from H. muticus leaf explants cultured on MS containing 0.5 mg/l BAP combined with 1mg/l 2, 4-D and incubated for 4 weeks at 25ºC under day/night condition. 3rd part, the transformed hairy root clone induced from the strain LBA 9402/12 showed the highest concentrations of hyoscyamine & scopolamine as compared to the concentration of the other plant parts or induced callus.

Alia Amer is a researcher at Agriculture Research Center from 2008 at medicinal and aromatic plants section. Got her MSc from Faculty of Agriculture, Cairo University in 2009, and PhD in 2015. She is interested in improving medicinal and aromatic plant yield and quality. She is part of a team involved in disseminating the improved technology.