Peptide Arrays on Membrane Supports von Joachim Koch/Michael Mahler

Detailed step-by-step protocols, including troubleshooting sections and many hints and comments from the authorsThe technology described is ideally suited to cope with the flood of data derived from the Human Genome ProjectThe protocols are well established and are provided by authors with plenty of experience with this technology

Inhaltsangabe1 SPOT Synthesis - Scope of Applications.- 2 Chemistry of Fmoc Peptide Synthesis on Membranes.- 3 Manual Peptide Synthesis.- 4 Automated Synthesis of Solid-Phase Bound Peptides.- 5 Epitope Mapping of Antibodies with Solid-Phase Oligopeptides.- 6 Protein-Protein Interactions.- 7 Analysis of Protein-DNA Interactions.- 8 Affinity Purification and Competition Assays Using Solid-Phase Oligopeptides.- 9 Mutational Analysis and Structure Predictions.- 10 Modification of Immobilized Peptides.- 11 Immobilized Peptides to Study Protein-Protein Interactions - Potential and Pitfalls.- Abbreviations.